To be played on 16th/17th January 2021

16th January 2021 14:00 EDF Challenge Cup N/A St Albans City DFC vs Farsley Celtic DFC
17th January 2021 14:00 EDF Challenge Cup N/A Washington DFC vs AFC Darwen Deaf
17th January 2021 14:00 EDF Challenge Cup N/A Birmingham DFC vs Blackburn Rovers DFC
17th January 2021 14:00 EDF Challenge Cup N/A St John's DFC vs Reading DFC
17th January 2021 14:00 EDF Challenge Cup N/A Welling United DFC vs Bristol City DFC
17th January 2021 14:00 EDF Challenge Cup N/A Derby DFC vs Bye
17th January 2021 14:00 EDF Challenge Cup N/A Bradford City DFC vs Brighton & Hove ADFC
17th January 2021 14:00 EDF Challenge Cup N/A Barnet DFC vs Fulham DFC