Played on 24th/25th April 2021

24th April 2021 W - L EDF Challenge Cup N/A Washington DFC vs AFC Darwen Deaf
24th April 2021 L - W EDF Challenge Cup N/A Birmingham DFC vs Blackburn Rovers DFC
24th April 2021 W - L EDF Challenge Cup N/A St John's DFC vs Reading DFC
24th April 2021 W - L EDF Challenge Cup N/A Welling United DFC vs Bristol City DFC
24th April 2021 W - L EDF Challenge Cup N/A Derby DFC vs Bye
24th April 2021 L - W EDF Challenge Cup N/A Bradford City DFC vs Brighton & Hove ADFC
25th April 2021 1 - 5 EDF Challenge Cup N/A St Albans City DFC vs Farsley Celtic DFC
25th April 2021 1 - 2 (AET) EDF Challenge Cup N/A Barnet DFC vs Fulham DFC